METABOLISM OF RIBOSE-1-C14 BY CELL-FREE EXTRACTS OF YEAST
نویسندگان
چکیده
منابع مشابه
Metabolism of ribose-1-C14 by cell-free extracts of yeast.
The fermentation of ribose-5-phosphate by cell-free extracts of yeast is characterized by the equimolar formation of COZ, ethyl alcohol, and inorganic phosphate (1). Dickens (1) and Sable (2) suggested from their studies of pentose phosphate metabolism by cell-free yeast preparations that fermentation proceeded in the following manner: (1) cleavage of ribosed-phosphate to a 2-carbon fragment an...
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In studies (l-3) on the synthesis in vivo of ribose by the tracer technique, it was desirable to have a rapid means of degrading the pentose for determination of the Cl4 distribution. The fermentation of pentose by Lactobacillus pentosus 124-2 seemed suitable for this purpose. Fred et al. (4) showed that this organism produces 1 mole each of lactic and acetic acids per mole of pentose utilized....
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A simple method for preparing chromatin assembly extracts has not been available for budding yeast. Here I describe such a method in detail. The assembly extract, a crude 100,000g supernatant, is prepared from cells disrupted in a manual or motorized grinder while they are frozen. The core histones and all soluble protein factors required for chromatin assembly under physiological conditions ar...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1955
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)57166-6